Juddmonte Farms have announced that Frankel will stand at stud for a fee of £125,000 for his first season as a stallion.
Rated as the best racehorse in the world after ending his career unbeaten in 14 races, he will be based at owner Khalid Abdullah's Banstead Manor Farm in Newmarket.
He will be joined on the roster by the former Roger Charlton-trained sprinter Bated Breath, who will command a fee of £8,000.
Philip Mitchell, Juddmonte Farms' general manager at Banstead Manor, said: "We believe our stallion roster is the best we have been able to offer breeders - and we are very excited with both of our new arrivals.
"The fee for Frankel reflects his merits as being perhaps the greatest racehorse we have ever witnessed.
"He is by Galileo, who is now recognised as the best sire in the world, and out of Kind, a winner of six races (from five to seven furlongs) and herself a daughter of one of the most influential stallions in the history of thoroughbred breeding, Danehill.
"Bated Breath will give breeders the opportunity to use a most genuine sprinter who was placed second in four Group One races. Ironically, his father Dansili started his career at the same fee and he also just failed to win a Group One race."
Dansili and Oasis Dream, Juddmonte's two previous standard bearers, will stand for £80,000.
"We have made minor adjustments to the fees of our two major stalwarts Dansili and Oasis Dream as we believe both of these world-class sires to be equally successful," said Mitchell.
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